Production of embryogenic callus from leaf explants of Camellia sinensis (L.)
نویسندگان
چکیده
منابع مشابه
Biochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.
Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...
متن کاملEffect of Medium Supplements on Agrobacterium rhizogenes Mediated Hairy Root Induction from the Callus Tissues of Camellia sinensis var. sinensis
Tea (Camellia sinensis L.) is recalcitrant to Agrobacterium-mediated genetic transformation largely due to the bactericidal effects of tea polyphenols and phenolics oxidation induced by necrosis of explant tissue over the process of transformation. In this study, different antioxidants/adsorbents were added as supplements to the co-cultivation and post co-cultivation media to overcome these pro...
متن کاملSomatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0...
متن کاملCallus Induction and Shoot Regeneration from Epicotyl Explants of Ethnomedicinally Important Caesalpinia bonduc (L.) Roxb
An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...
متن کاملAntioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production.
The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide ani...
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ژورنال
عنوان ژورنال: Journal of the National Science Foundation of Sri Lanka
سال: 2007
ISSN: 2362-0161,1391-4588
DOI: 10.4038/jnsfsr.v35i3.2018